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Biochemistry- Osazone Formation Mechanism
Osazone is the derivative of carbohydrates that are formed by the reaction of carbohydrates with phenylhydrazine. As aldehyde, aldoses react with phenylhydrazine to form phenylhydrazones. In 1875, Emil Fischer found that reducing benzene diazonium chloride by sulfur dioxide yields phenylhydrazine. Fischer reported that the phenylhydrazine he had discovered could be used as a powerful tool in the study of carbohydrates. If an excess of phenylhydrazine is used, the reaction proceeds further to yield products known as osazones.
